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KMID : 0613820020120040577
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Journal of Life Science
2002 Volume.12 No. 4 p.577 ~ p.583
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The Growth of Transgenic Tobacco’s Suspension Culture and the Production of β-Glucuronidase in Bubble Column Bioreactor
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Kim Seok-Woo
Lee Dong-Geun
Hyun Jin-Won
Lee Sang-Hyun
Ha Jong-Myuong
Lee Jae-Hwa
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Abstract
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The growth kinetics and the production of β-glucuronidase from transgenic tobacco’s suspension culture was investigated in the flask culture and a 2.5 L bubble column reactor. The growth of bubble column reactor was similar to that of flask culture. However, in the bubble column reactor, the production of β-glucuronidase reached 2850 U/mg (85-fold higher than that of flask culture). In both case, the production level of β-glucuronidase was fluctuated, which was resulted from periodical degradation of the protein. Sucrose is important component in plant culture medium. Twice addition of sucrose in bubble column reactor could not improve cell growth, since other components in a medium were already depleted. However, the addition of sugar decreased cell size, which facilitated the operation of bioreactor. The production of β-glucuronidase was continuously increased, however final concentration of β-glucuronidase was similar to that without sucrose addition.
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KEYWORD
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plant suspension culture, β-glucuronidase, bubble column bioreactor, transgenic plant
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